Lionheart, W. R. B. (2015)

OMERO workshop

TOPIM TECH
Chania, July 2018
Will Moore, Petr Walczysko

Outline

  • What is OME?
  • What is OMERO?
  • Workshop overview

OME: Interoperability

OMERO is a server with clients

Image Data - Read by Bio-Formats

  • Support for reading > 150 image formats
  • Read pixel data and metadata
  • Includes 5D images, HCS data, Tiled WSI images
  • Domains: biological, medical, general (tiff, png etc)

Metadata

  • Stored in Relational Database
  • Acquisition metadata
  • User-added Annotations
  • ROIs / Segmentations

Search

  • Text and Annotations indexed with Lucene
  • Allows keyword searches

OMERO.tables

  • HDF5 tables for analysis results
  • Flexible columns

OMERO API

Python | Java | Matlab | R

Different clients for different jobs

OMERO clients

  • OMERO.insight
  • OMERO.webclient
    • Browsing images
    • Viewing metadata
    • Data management
    • Searching
  • OMERO web apps
    • OMERO.iviewer
    • OMERO.figure
    • OMERO.parade

OMERO.iviewer

OMERO.parade

OMERO.web framework is extensible

Open with... Web apps

OMERO Python scripts

Fiji

CellProfiler and R in Jupyter notebooks

Orbit

Tomorrow's workshop

Tomorrow's workshop

  • Please bring your laptops
  • We have an OMERO server prepared:
    • User accounts set-up
    • Sample data already imported
    • Data from IDR: idr0002 and idr0021
  • Everything is web-based except:

Thank you