Lionheart, W. R. B. (2015)
OMERO workshop
TOPIM TECH
Chania, July 2018
Will Moore, Petr Walczysko
Outline
What is OME?
What is OMERO?
Workshop overview
OME: Interoperability
OMERO is a server with clients
Image Data - Read by Bio-Formats
Support for reading > 150 image formats
Read pixel data and metadata
Includes 5D images, HCS data, Tiled WSI images
Domains: biological, medical, general (tiff, png etc)
Metadata
Stored in Relational Database
Acquisition metadata
User-added Annotations
ROIs / Segmentations
Search
Text and Annotations indexed with Lucene
Allows keyword searches
OMERO.tables
HDF5 tables for analysis results
Flexible columns
OMERO API
Python | Java | Matlab | R
Different clients for different jobs
OMERO clients
OMERO.insight
OMERO.webclient
Browsing images
Viewing metadata
Data management
Searching
OMERO web apps
OMERO.iviewer
OMERO.figure
OMERO.parade
OMERO.iviewer
OMERO.parade
OMERO.web framework is extensible
Open with... Web apps
OMERO Python scripts
Fiji
CellProfiler and R in Jupyter notebooks
Orbit
Tomorrow's workshop
Tomorrow's workshop
Please bring your laptops
We have an OMERO server prepared:
User accounts set-up
Sample data already imported
Data from IDR:
idr0002
and
idr0021
Everything is web-based except:
Fiji. Google
Fiji OMERO
Orbit.
www.orbit.bio
Thank you