OMERO workshop

Dundee, November 2018
Jean-Marie Burel

Outline

  • Intro: OME
  • What is OMERO?
  • Workshop: Using OMERO

A typical microscopy workflow

OME: Interoperability

OMERO is a server with clients

Image Data - Read by Bio-Formats

  • Support for reading > 150 image formats
  • Read pixel data and metadata
  • Includes 5D images, HCS data, Tiled WSI images
  • Domains: biological, medical, general (tiff, png, etc.)

Metadata

  • Stored in a Relational Database
  • Acquisition metadata
  • User-added Annotations
  • ROIs / Segmentations

Search

  • Text and Annotations indexed with Lucene
  • Allows keyword searches

OMERO.tables

  • HDF5 tables for analysis results
  • Flexible columns

OMERO API

Python | Java | Matlab | R

OMERO.web framework is extensible

Different clients for different jobs

OMERO clients

  • OMERO.insight
  • OMERO.webclient
    • Browsing images
    • Viewing metadata
    • Data management
    • Searching
  • OMERO web apps
    • OMERO.iviewer
    • OMERO.figure
    • OMERO.parade
    • ...
  • ...

OMERO.iviewer

OMERO.figure

OMERO.parade

Open with... Web apps

Integration

Set up

Own Data

Each others data – read-annotate setup

OMERO in Dundee

  • Production server:
    • nightshade.openmicroscopy.org
    • Free access
  • Use LDAP credentials

Thank you

Today's Demo...

outreach.openmicroscopy.org

(Server located in Dundee)

openmicroscopy.org

Google: 'OMERO Demo'
to sign up for a demo account