<?xml version="1.0" encoding="UTF-8"?><ProtocolTitle xmlns="http://www.openmicroscopy.org/Schemas/Editor/2008-04" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" elementName="Seed Cells" protocolFileName="SeedCells.pro.xml" version="3.0-3.0" xsi:schemaLocation="http://www.openmicroscopy.org/Schemas/Editor/2008-04 http://www.openmicroscopy.org/Schemas/Editor/2008-04/Editor.xsd"><DateTimeField alarmSeconds="32400" elementName="Seed Cells"/><FixedStep elementName="Procedure" fieldLockedUTC="1210329584075" fieldLockedUserName="will" lockLevel="templateLocked" substepsCollapsed="false"><FixedStep description="Defrost Trypsin." elementName="Warm PBS &amp;amp; Media to 37'C"/><FixedStep description="Rinse cells 3x in warm PBS." elementName="Wash cells in PBS"/><NumberField default="5" description="Add trypsin, then remove and add another few ml. Remove and incubate, &#10;      with shaking, till cells start to come off the dish." elementName="Add trypsin" units="ml"/><NumberField default="10" description="This will stop the trypin reaction." elementName="Resuspend in media" units="ml" value="5"/></FixedStep><TextField default="HeLa" elementName="Cell Line" substepsCollapsed="true"><TextBox description="Notes about the cells: &#10;    &#10;    &lt;br&gt;&#10;      ie Are they stably transfected?&#10;    &#10;    &lt;br&gt;&#10;      Where they came from, etc." elementName="Description"/><NumberField description="The number of times the cells have been passaged since they started &#10;      growing." elementName="Passage Number" units="passages"/></TextField><DropDownMenu default="24-well plate" dropdownOptions="6-well plate, 24-well plate, 10 cm dish, 6 cm dish, 35 mm MatTech dish" elementName="Plate format" substepsCollapsed="false" value="6-well plate"/></ProtocolTitle>