<?xml version="1.0" encoding="UTF-8"?><ProtocolTitle xmlns="http://morstonmud.com/omero/schemas" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" elementName="Live Cell Imaging" protocolFileName="Live-Cell-Imaging.pro.xml" version="3.0-3.0" xsi:schemaLocation="http://morstonmud.com/omero/schemas http://morstonmud.com/omero/schemas/PE.xsd"><DateTimeField UTCMillisecs="1209942000000" alarmSeconds="32400" elementName="Seed HeLa" substepsCollapsed="true"><DropDownMenu dropdownOptions="H2B-GFP, CENP-A-GFP, tubulin-RFP" elementName="Cell Line" value="H2B-GFP"/><DropDownMenu dropdownOptions="6-well plate, 24-well plate, MatTech 35mm" elementName="Plate Format" value="6-well plate"/></DateTimeField><DateTimeField UTCMillisecs="86400000" alarmSeconds="-3600" elementName="Drug treatment" seconds="39600" substepsCollapsed="true"><TextField elementName="Drug name"/><NumberField elementName="Drug Concentration" units="ug/ml"/></DateTimeField><DateTimeField UTCMillisecs="86400000" elementName="Live cell imaging" seconds="43200" substepsCollapsed="false"><FixedStep elementName="&#10;      Imaging Parameters&#10;    &#10;  " substepsCollapsed="false"><TextField elementName="&#10;      Microscope&#10;    &#10;  " substepsCollapsed="true" value="2nd Floor DV"><TextBox elementName="&#10;      Comments&#10;    &#10;  " value="Held MatTech dish to stage with tape onto lid. Mostly didn't move too much.&#10;Aimed for wide a field as possible (max pixels with this binning).&#10;Z-resolution OK for seeing chromosomes, while limiting light exposure.&#10;Took FITC and &quot;trans&quot; image with same filters.&#10;Chose fields for reasonable cell density with plenty of mitotic cells. Although these were too far gone by the time&#10;imaging started, still a good marker for area where other cells might divide.&#10;Cells seemed to keep dividing OK, with these levels of light."/></TextField><TextField default="40 X" elementName="&#10;      Lens&#10;    &#10;  " value="40 X"/><NumberField default="568" elementName="&#10;      Pixels-X&#10;    &#10;  " units="pixels" value="568"/><NumberField default="568" elementName="&#10;      Pixels-Y&#10;    &#10;  " units="pixels" value="568"/><DropDownMenu default="x 2" dropdownOptions="x 1,x 2,x 4" elementName="&#10;      Binning&#10;    &#10;  " value="x 2"/><NumberField default="6" elementName="&#10;      Z-planes&#10;    &#10;  " units="planes" value="6"/><NumberField default="1.5" elementName="&#10;      Z-spacing&#10;    &#10;  " units="uM" value="1.5"/><TextField default="FITC ex/em Quad mirror" elementName="&#10;      Filter set&#10;    &#10;  " value="FITC ex/em Quad mirror"/><DropDownMenu default="33%" dropdownOptions="none, 33%, 50%" elementName="&#10;      ND filer&#10;    &#10;  " value="33%"/><NumberField default="0.1" elementName="&#10;      Exposure duration&#10;    &#10;  " units="secs" value="0.1"/><TimeField default="00:05:00" elementName="&#10;      Time interval&#10;    &#10;  " value="00:05:00"/><TimeField default="04:00:00" elementName="&#10;      Total imaging time (approx)&#10;    &#10;  " value="04:00:00"/><NumberField default="30" elementName="&#10;      Number of fields per sample (approx)&#10;    &#10;  " units="fields" value="30"/></FixedStep></DateTimeField></ProtocolTitle>