The Image Data Resource

Simon Li

GRE seminar, 6 December 2016

Overview

  • Why did we build this?
  • How did we create it?
  • What can you do with it?

The Image Data Resource

Why did we build this?

Open science: Open access + Open data

Open science: Open access + Open data

  • Reproducibility
  • Re-use / re-analysis
  • Combine with other data sources

Open science: Open access + Open data

Problems with imaging data

  • Storage and management
  • Discoverability

Contextual metadata is critical

How did we create it?

The Image Data Resource: in numbers

  • 25 published studies
  • 42 TB data
  • 14 million files
  • 1 million experiments
  • Computational resources:
    • 32 CPUs
    • 128 GB memory

The Image Data Resource: data management

  • All studies are curated
  • Data is arranged in a common layout
  • Cross referenced with:
    • External resources
    • Other IDR studies

What can you do with it?

Browse the data

Raw data not previously accessible, for example Mitocheck (published 2010)

External links: studies

External links: logbooks

External links: genes

Internal links: genes

IDR: A curated collection of diverse cross-referenced studies

  • Compare genes and phenotypes across different studies/experiments using MAPR, a new OMERO.web application.
    • Genes (19,598)
    • Phenotypes (151)
    • siRNAs
    • Compounds
    • Organisms

Genes

Phenotypes

  • Elongated cell phenotype
  • S. pombe (idr-0001 Sysgro)
  • HeLa (idr-0008 Actinome)
  • HeLa (idr-0012 CellMorph)
  • Elongated cell phenotype
  • S. pombe (idr-0001 Sysgro)
  • HeLa (idr-0008 Actinome)
  • HeLa (idr-0012 CellMorph)
Histone methyltransferase (HMT)

Phenotypes in the IDR

The Image Data Resource: in numbers

  • 25 published studies
  • 42 TB data
  • 14 million files
  • 1 million experiments
  • 19,598 genes
  • 151 Phenotypes

Analysis platform

Next steps

The IDR team

Jason Swedlow
Josh Moore
Simon Li
Eleanor Williams
Gabriella Rustici
Aleksandra Tarkowska
Richard Ferguson
Simone Leo
Alvis Brazma
Ugis Sarkans
Simon Jupp
Tony Burdett
Rafael Carazo-salas
Bálint Antal
Anatole Chessel